Avaliação da atividade antibacteriana e esporicida dos óleos essenciais de copaifera multijuga, thymus vulgaris e nisina frente á alicyclobacillus acidoterrestris

Silva, Angela Aparecida da

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Campo DC	Valor	Idioma
dc.contributor.advisor	Benício Alves de Abreu Filho	pt_BR
dc.contributor.author	Silva, Angela Aparecida da	pt_BR
dc.date.accessioned	2018-04-05T18:05:01Z	-
dc.date.available	2018-04-05T18:05:01Z	-
dc.date.issued	2015	pt_BR
dc.identifier.uri	http://repositorio.uem.br:8080/jspui/handle/1/1452	-
dc.language	por	pt_BR
dc.publisher	Universidade Estadual de Maringá	pt_BR
dc.rights	openAccess	pt_BR
dc.subject	Alicyclobacillus acidoterrestris	pt_BR
dc.subject	Óleos essenciais	pt_BR
dc.subject	Atividade antibacteriana	pt_BR
dc.subject	Copaifera multijuga	pt_BR
dc.subject	Thymus vulgaris	pt_BR
dc.subject	Nisina	pt_BR
dc.subject	Micro-organismo termorresistente	pt_BR
dc.subject	Avaliação dos agentes antibacterianos	pt_BR
dc.subject	Micro-organismo deteriorante	pt_BR
dc.subject	Suco de laranja	pt_BR
dc.subject	Segurança alimentar	pt_BR
dc.subject	Suco alimentar	pt_BR
dc.subject	Antibacterianos	pt_BR
dc.subject	Brasil.	pt_BR
dc.subject	Antibacterial	en
dc.subject	Copaifera multijuga	en
dc.subject	Thymus vulgaris	en
dc.subject	Alicyclobacillus acidoterrestris	en
dc.subject	Orange juice	en
dc.subject	Brazil.	en
dc.title	Avaliação da atividade antibacteriana e esporicida dos óleos essenciais de copaifera multijuga, thymus vulgaris e nisina frente á alicyclobacillus acidoterrestris	pt_BR
dc.title.alternative	Evaluation of the antibacterial and sporicidal activity of the essential oils of Copaifera multijuga and Thymus vulgaris and nisin against Alicyclobacillus acidoterrestris	en
dc.type	masterThesis	pt_BR
dc.contributor.referee1	João Alencar Pamphile - UEM	-
dc.contributor.referee2	Jane Martha Graton Mikcha - UEM	-
dc.description.resumo	: This study aimed to evaluate the bactericidal, bacteriostatic and sporicidal activity of the essential oils (EOs) of Copaifera multijuga and Thymus vulgaris against Alicyclobacillus acidoterrestris. The combined activity of the EOs and nisin was also assessed by determining the fractional inhibitory concentration (FIC). In addition, the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum sporicidal concentration (MSC) were evaluated by serial microdilution in Bacillus acidoterrestris (BAT) broth with subcultures in BAT agar. The MIC of the C. multijuga and T. vulgaris EOs were 300 μg mL-1 and 500 μg mL-1, respectively, and the MBC of both EOs was >1,000 μg mL-1. The best MSC of C. multijuga was reached at a concentration of 500 μg mL-1 with a reduction of 3.07 log CFU mL-1. For the EO of T. vulgaris, better results were observed at concentrations higher than 500 μg mL-1, and a reduction of 2.05 log CFU mL-1 was achieved at 1,000 μg mL-1. The checkerboard method showed that the combination of EOs and nisin had an additive interaction (FIC index of 0.75). The bactericidal activity was confirmed by the death curve. According to the selectivity index, the treatment was less selective for A. acidoterrestris than for Vero cells. The flow cytometry results indicated that the vegetative forms of A. acidoterrestris had a higher incidence of vegetative cells with alterations in cell membrane integrity compared to their spores; however, we cannot conclude that this is the main mechanism of action of the EOs. Our studies showed that the tested substances had good antibacterial properties because they reduced the viability of A. acidoterrestris. Thus, it is evident that the EOs of C. multijuga and T. vulgaris have potential for use as antibacterial agents against A. acidoterrestris.	pt_BR
dc.publisher.country	Brasil	pt_BR
dc.publisher.program	Programa de Pós-Graduação em Ciência de Alimentos	pt_BR
dc.publisher.initials	UEM	pt_BR
dc.subject.cnpq1	Ciências Agrárias	pt_BR
dc.publisher.local	Maringá, PR	pt_BR
dc.description.physical	46 f	pt_BR
dc.subject.cnpq2	Ciência e Tecnologia de Alimentos	pt_BR
dc.publisher.center	Centro de Ciências Agrárias	pt_BR
Aparece nas coleções:	2.1 Dissertação - Ciências Agrárias (CCA)

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