1. RI-UEM
  2. 2. Dissertações
  3. 2.1 Dissertação - Ciências Agrárias (CCA)
Use este identificador para citar ou linkar para este item: http://repositorio.uem.br:8080/jspui/handle/1/1476
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dc.contributor.advisorJane Martha Graton Mikchapt_BR
dc.contributor.authorYassunaka, Natália Norikapt_BR
dc.date.accessioned2018-04-05T18:08:31Z-
dc.date.available2018-04-05T18:08:31Z-
dc.date.issued2015pt_BR
dc.identifier.urihttp://repositorio.uem.br:8080/jspui/handle/1/1476-
dc.description.abstracten
dc.languageporpt_BR
dc.publisherUniversidade Estadual de Maringápt_BR
dc.rightsopenAccesspt_BR
dc.subjectEscherichia coli produtora de toxina Shiga (STEC)pt_BR
dc.subjectGenes de virulênciapt_BR
dc.subjectBactérias patogênicaspt_BR
dc.subjectEritrosinapt_BR
dc.subjectDerivadospt_BR
dc.subjectFotoinativaçãopt_BR
dc.subjectBactérias deteriorantespt_BR
dc.subjectBrasil.pt_BR
dc.subjectPhotoinactivationen
dc.subjectErythrosineen
dc.subjectEster derivativesen
dc.subjectLight-emitting diodeen
dc.subjectBacteriaen
dc.subjectBrazil.en
dc.titlePhotodynamic inactivation mediated by erythrosine and its derivaties on foodborne pathogens and spoilage bacteriapt_BR
dc.title.alternativePhotodynamic inactivation mediated by erythrosine and its derivaties on foodborne pathogens and spoilage bacteriaen
dc.typemasterThesispt_BR
dc.contributor.referee1Benício Alves de Abreu Filho - UEM
dc.contributor.referee2Elza Kimura - UEM
dc.description.resumo: The purpose of the present study was to evaluate the efficacy of photodynamic inactivation (PDI) mediated by erythosine (ERY) and its ester derivatives erythrosine methyl ester (ERYMET) and erythrosine butyl ester (ERYBUT) on foodborne pathogens and spoilage bacteria. We evaluated Staphylococcus aureus ATCC 25923, Aeromonas hydrophila ATCC 7966, Salmonella enterica serotype Typhimurium ATCC 14028, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. The toxicity of all of the compounds was assessed in VERO cells. Photodynamic inactivation mediated by ERY and its derivatives combined with a light-emitting diode (LED) was performed at different concentrations and exposure times. S. aureus was more photosensitive than Gram-negative bacteria to ERY, ERYMET, and ERYBUT. The ERY-mediated PDI of S. aureus induced a significant reduction of 4.0 log CFU/ml at a light dose of 40 J/cm2. ERYMET and ERYBUT at lower light doses than ERY completely eradicated S. aureus. When photoirradiated with ERY at light doses of 156 and 234 J/cm², A. hydrophila was completely eradicated. ERYBUT was more efficient in the PDI of A. hydrophila than ERYMET, even at 1 x 10-5 M and lower light doses. S. Typhimurium, E. coli, and P. aeruginosa required higher concentrations of photosensitizers to reduce cell survival. ERYBUT and ERY may be promising photosensitizing agents against A. hydrophila and S. aureus. They were effective at reducing bacterial counts at non-toxic concentrations. The photoinactivation rate of the evaluated bacteria decreased in the following order: S. aureus > A. hydrophila > E. coli > S. Typhimurium > P. aeruginosa.pt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.programPrograma de Pós-Graduação em Ciência de Alimentospt_BR
dc.publisher.initialsUEMpt_BR
dc.subject.cnpq1Ciências Agráriaspt_BR
dc.publisher.localMaringá, PRpt_BR
dc.description.physical34 fpt_BR
dc.subject.cnpq2Ciência e Tecnologia de Alimentospt_BR
dc.publisher.centerCentro de Ciências Agráriaspt_BR
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