Distribuição dos genótipos de HPV e integração dos HPV 16 e 18 em mulheres da região sul do Paraná/Brasil com anormalidades cervicais

Abstract

The identification of high-risk (HR) Human Papillomavirus (HPV) types offers the possibility of improving the efficiency of cervical screening programs. Therefore, there is a continuing need for the identification of factors that modulate the risk of disease progression, and which might serve as predictive biomarkers. The aim of the present study was determine by polymerase chain reaction (PCR) the distribution of HPV genotypes and the E2 gene disruption of the HPV16/18 to assess whether viral pure integration is correlated with the degree of cervical abnormalities in South Brazilian women. A total of 137 women were studied between November 2009 and August 2011, cervical samples were collected in the colposcopy clinic at União da Vitória city, south/Brazil. The mean age was 33.60±30.72 years (range of 16-78 years), and those with invasive carcinoma (IC) were older (p < 0.05). The prevalence of total DNA- HPV, HR-HPV and multiple infections in all women was 56.2%, 38.7% and 12.4%, respectively and were higher detected in those with high-grade lesions (HSIL) and invasive carcinomas (IC) (p < 0.05). The most common HPV detected types was HPV-16 (17.5%; p < 0.05). The low-risk (LR)-HPV types were detected in 29.2%, being HPV-72 the most common (15.3%) and HPV-6 and -11 were not detected. For multiple infections, the most common occurred between HPV-72 and 16 (64.7% the all co-infections and 8.0% of the women). The overall HPV-18 E2 gene disruption was 100.0% and this infection was evenly distributed in atypia (ASC-H), low-grade lesions (LSIL), HSIL and IC (0.7% each). For HPV-16 E2 gene, the overall disruption was 54.2%, and occurred in 50.0% in ASC, 66.7% in LSIL, 53.8% in HSIL and 50% in IC (p < 0.05). Considering the similar results for pure integration of HPV-16 among different degrees of cytological abnormalities since ASC to IC, the use of PCR does not seem to be applicable to distinguish women with greater propensity for progression of the lesions.