Obtenção e caracterização de nanopartículas contendo ácido ascórbico utilizando o subproduto da extração de própolis

Abstract

Among the research conducted in the pharmaceutical field, the particulate delivery systems, especially micro/nanoparticles, have attracted great interest in recent decades; they allow the administration of drugs by different routes. These systems have permitted an increase in therapeutic efficacy, reduction in the incidence of side effects, an adequate supply of the drug for a long period, with reduced frequency of doses that has facilitated patients' adherence to treatment. Thus, various polymers having the most different origins have been studied and used in the process of micro/nanoencapsulation. Propolis is a strongly adhesive substance, widely used by the population. During the pursuit of his statement, there is the formation of bud-resinous residue, which has shown the ability to form particles. Accordingly, to evaluate the kinetics of release from nanoparticles (NP), was used ascorbic acid (AA), as a model drug, a cofactor of numerous physiological reactions, which has an antioxidant action. Therefore, this study aimed to obtain and characterize nanoparticles by-product (SP) extraction of propolis containing AA. Quantification of vitamin C was performed by high performance liquid chromatography (HPLC). We carried out a quality control of the propolis extract and 30% (w / w). The BP had obtained their characteristics evaluated for their ability to structure. The nanoparticles were obtained from spray drying. The antioxidant activity of AA was determined by DPPH and ABTS. The method of quantification of AA by HPLC was shown to be valid. Both as propolis extract and ascorbic acid met the quality standards. The BP has been considered appropriate to develop particulate structures. The spherical nanoparticles showed up, but with morphological variation and size, some agglomerations and average diameter between 110.93 and 298.53 nm and polydispersity index ranging from 0.444 to 0.797. The technology of micro/nanoencapsulation enabled a good encapsulation efficiency, with a slow release, not governed by Fickian diffusion. The antioxidant activity of NP evaluated by ABTS method showed an enhancement of antioxidant capacity of AA. Thus, it has been shown to be possible to use the by-product of the extraction of propolis for the development of nanoparticles, which could provide modified release of Vitamin C.