Capacidade de migração sobre matriz dérmica acelular por Candida spp. E Rhodotorula sp. isoladas de pele

Abstract

In recent decades, the burned patient prognosis showed great improvement with the development of special care, early debridement and antibiotic broad spectrum therapy, but remains considerable morbidity and mortality, and infectious complications the main causes. The acellular dermal matrix (ADM) is a completely acellular artificial device which has the function of controlling the water loss, prevent penetration of microorganisms and allows migration of endothelial cells and fibroblasts to patients matrix. This study aimed to evaluate the capacity for colonization and penetration of Candida spp. and Rhodotorula sp. in dermal matrix used in the restoration of dermis in burned patients. They were evaluated three yeasts isolated from patients with burns and skin scales, identified as Candida parapsilosis, Candida glabrata and Rhodotorula mucilaginosa. The minimum inhibitory concentration (MIC) was evaluated for fluconazole and amphotericin B have also verified the capacity of penetration of the dermal matrix by Forming Units Method Colonies (CFU); epifluorescence microscopy and scanning electron microscopy. It was observed that Candida isolates were susceptible to fluconazole, while R. mucilaginosa was tough. Front amphotericin B, C. parapsilosis and R. mucilaginosa were sensitive, while C. glabrata showed a high value of MIC. In 72 hours, all isolates had the ability to cross the ADM. C. parapsilosis showed the lowest growth, while R. mucilaginosa had an intermediate and C. glabrata the highest growth. All plates were incubated for seven days, the growth of C. glabrata and C. parapsilosis increased by 1 log over the threeday test. This is the first study that demonstrates the ability of colonization and penetration saprophytic yeast and from the human microbiota in an acellular dermal regeneration device. All isolates showed colonization and penetration of dermal device, creating a potential risk of burn patients develop wound infections which may develop in the sepsis and even death.