Efeito do álcool etílico diluído e dinamizado em camundongos infectados pela cepa Y de Trypanossoma cruzi

Abstract

Evaluate the effect of diluted and dynamized ethanol in different potencies under the clinical and parasitological parameters of Swiss mice infected by Trypanosoma cruzi Y strain. The experiment was conducted as a blind assay, controlled, randomized by draw. It was approved by the Ethics Committee on Animal Use of Universidade Estadual de Maringá (062/2014). The study involved 80 male Swiss mice, eight weeks old, wherein, 68 animals were intraperitoneally inoculated with 1400 typomastigotes blood forms of the T. cruzi Y strain and divided in groups: IC - 17 animals infected and untreated; Three groups of 17 animals each treated with grain alcohol diluted and dynamized in different potencies: 1CH (1: 101), 6CH (1: 1012) and 30 CH (1: 1060). Another group of 12 uninfected animals and untreated (CNI - healthy control group), maintained under the same conditions, was used for comparison of clinical parameters. All treatments were offered ad libitum, 48 hours before and after the infection, followed by doses every 56 hours, until the 9th day after infection, for 16 uninterrupted hours. Were evaluated: Clinical parameters (weight, temperature, water, feed and production of excreta); Parasitological parameters (pre-patent period, patent period, peak maximum of the parasitaemia, total parasitaemia and average parasitaemia during the curve); Survival time and Mortality rate. The significance level was set at p<0.05. The dynamized ethyl alcohol presented measurable biological effects on murine T. cruzi infection. The different potencies tested (1CH, 6CH and 30CH) induced different effects in the infected mice. The effects were more beneficial in 1CH potency, intermediaries for 6CH and harmful in 30CH. The lower potency (1CH) brought the best prognosis clinical, with increased weight and maintaining the temperature (p<0.05), increased water consumption, feed and excreta production compared to the IC, and parasitological, with the lowest parasitemia rates (maximum peak, average parasitaemia during the curve and total parasitaemia), reflecting significantly higher survival time (p<0.05) compared to 30cH and IC. The treatment with the higher potency (30CH) worsened the clinical condition of the animals with the highest numbers of the parasitological parameters, resulting in higher mortality and lower survival time. The 6CH potency promoted intermediate effects to the previous two potencies without clinical and parasitological 33 improvement but with significantly higher survival time (p<0.05) than the mice in the control group of infection, not excluding the possibility of it could influence some other important marker of resistance, not identified on this study. The dynamized ethyl alcohol changes the natural course of infection by T. cruzi, and can not be considered as an inert ingredient in homeopathic preparations used herein. The results suggest that the effect observed for active ingredients diluted in alcohol should be carefully analyzed, although the alcohol or the active ingredient mixture more alcohol interfere these effects, requiring the inclusion of untreated controls in experimental protocols in which the alcohol has been used as control.