Desenvolvimento de filmes micro e nanoporosos de poliestireno utilizando o método breathfigure : aplicação como scaffolds em cultura celular

Abstract

Ordered structures always promoted fascination and interest in the philosophers, mathematicians and artists as much as the math involved in their esthetic qualities. Currently, scientists of materials have been studying the applications that the ordered structures can provide, among the materials studied thin polymer films with porous surfaces with specific pattern have special attention due to its various applications. Several methods for obtaining porous surfaces, such as those using lithography techniques, methods based on self-assembly patterns and recently the method known as breath figure. The method breath figure permits the formation of well ordered hexagonal arrays on the surface of substrates by a process simple, quick, dynamic, economical and reproducible. By this method was possible to obtain porous film of polystyrene supported on various polymeric substrates. Factors like speed spin coating,substrate concentration of polystyrene and solvent influenced the formation, pore diameter and depth of the ordered structures of polystyrene. The rotations of the spin coating studied induced the formation of pores having average diameter between 1,2 μm to 202 nm with a roughness average 130 to 25 nm, respectively. The substrates studied promoted nanoporous films of PS with average diameter 507 to 123 nm and average roughness 132 to 6 nm, respectively. Porous films with average diameter of 346 nm and 256 nm and with roughness average of 96 and 22 nm, respectively, supported on poly (ethylene terephthalate) were evaluated as nanoscaffolds compared with Vero cells. The results showed higher cell viability compared to control consisting of polystyrene plate. This fact implies that the nanoscaffolds obtained of unprecedented way, are able to mimic the interactions that have cells with the nano-protein of extracellular matrix.