Desenvolvimento de modalidade cromatográfica baseada na identidade digital oxidativa de compostos orgânicos

Abstract

In this paper a new method based on chromatographic identididade digital oxidation of organic compounds has been developed enabling the separation and identification of organic compounds using liquid chromatography with spectrophotometric detectors. A sequential injection analysis (SIA) was used in conjunction with the Fenton reactions (Fe (II) and H2O2) to promote cleavage of organic compounds eluted from the chromatography column and how to manage the process of solid phase extraction for isolation/preconcentration, and re-introduction of the reaction products in the liquid chromatograph to obtain the identity oxidative. This approach combined liquid chromatography with photodiode array detectors (UV/ Vis) using the Fenton reactions for to promote the cleavage of compounds, allowing the development of a chromatographic of a method that is based on an initial separation of the organic constituents a sample by liquid chromatography followed by further separated after partial oxidation of the components of the sample, which achieves distinct peaks for the two chromatograms. First, original peaks of each sample constituent unoxidized and second, several peaks for each species should reflect that the previously formed products after the process of partial oxidation of the constituents of the sample, providing a set of data the products formed which was used to provide oxidative digital identity of each compound separately. At the end of the chromatographic process, the chromatograms provide information regarding the retention time of the analyte, which is related to the retention time of substances in standard solutions, UV/Vis spectrum of the compounds analysis (riboflavin and cyanocobalamin) , as well as signs of oxidation products for each species with their respective spectra and the relationship between the peak areas for each species formed which enabled the identification with higher reliability compounds analyzed. With the acquisition of digital identities of oxidative riboflavin and cyanocobalamin is possible to use the proposed method for the separation and identification of organic compounds that are eluted from a chromatographic column without the use of sophisticated and expensive equipment as the mass spectrometer and the nuclear magnetic resonance