Resinas infiltrantes experimentais : avaliação da sorção de água e do potencial de liberação de íons bioativos

Abstract

RESUMO: A minima intervencao tem sido base para o tratamento da carie dentaria, que continua sendo uma das principais patologia da infancia. Uma intervenção precoce e capaz de controlar a evolucao da doenca. O objetivo do presente trabalho e avaliar in vitro o desempenho de um infiltrante resinoso experimental contendo particulas de ceramica, com foco em suas propriedades de sorcao de agua, estabilidade e liberacao de ions bioativos. Metodos: Experimento 1. Resinas experimentais foram formuladas com monomeros de metacrilato, sistema iniciador, particulas ceramicas e agente antimicrobiano. A sorcao de agua e a solubilidade foram avaliadas conforme a ISO 4049:2019, utilizando especimes de 5x2 mm, polimerizados por 60 segundos e submetidos a ciclos de dessecacao e pesagem. As massas iniciais (M1), intermediarias (M2) e finais (M3) foram registradas para o calculo da sorcao de agua como a diferenca entre M2 e M1, e da solubilidade como a diferenca entre M1 e M3. Experimento 2: Resinas experimentais contendo particulas ceramicas foram formuladas e armazenadas em agua Milli-Q por 24 horas a 37oC. A solucao de armazenamento foi coletada e submetida a um teste colorimetrico para a quantificacao de ions calcio. Resultados: Experimento 1 - As resinas experimentais apresentaram sorcao de agua semelhante ao ICON, indicando estabilidade dimensional. No entanto, os grupos R6, R7 e R8 exibiramsolubilidade significativamente maior (Kruskall-Wallis; Dunn, p < 0,05), sugerindo menor durabilidade clinica. Experimento 2 - O teste colorimetrico nao apontou presenca de calcio nas amostras pertencentes aos grupos controle (0±0.06 ug/well) e experimentais apos 24 horas de armazenamento (ANOVA; p>0.05).. Conclusao: Conclui-se que, embora as resinas experimentais tenham demonstrado estabilidade dimensional, a alta solubilidade e a falta de bioatividade limitam seu uso como agentes remineralizadores. Ajustes na formulacao sao necessarios para melhorar a estabilidade quimica e o potencial bioativo.

ABSTRACT: Minimal intervention has been the cornerstone of dental caries treatment, which remains one of the main childhood diseases. Early intervention is capable of controlling the progression of the disease. The aim of this study is to evaluate the performance of na experimental resin infiltrant containing ceramic particles, with a focus on its water sorption properties, stability, and release of bioactive ions. Methods: Experiment 1: The experimental resins were formulated using methacrylate monomers, an initiating system, ceramic particles, and na antimicrobial agent. Water sorption and solubility were evaluated according to ISO 4049:2019, using specimens measuring 5×2 mm, polymerized for 60 seconds and subjected to cycles of desiccation and weighing. The initial (M1), intermediate (M2), and final (M3) masses were recorded to calculate water sorption as the difference between M2 and M1, and solubility as the difference between M1 and M3. Experiment 2: Experimental resins containing ceramic particles were formulated and stored in Milli-Q water for 24 hours at 37°C. The storage solution was collected and subjected to a colorimetric test to quantify calcium ions. Statistical analysis was conducted using the Shapiro-Wilk test to assess normality and homogeneity of variance. As the data did not exhibit a normal distribution, the Kruskal-Wallis test was applied, followed by Dunn's test for multiple comparisons, with a significance level of 5%. Results: Experiment 1: The experimental resins exhibited water sorption similar to ICON, indicating dimensional stability. However, groups R6, R7, and R8 showed significantly higher solubility (Kruskal-Wallis; Dunn, p < 0.05), suggesting lower clinical durability. Experiment 2: The colorimetric test did not detect the presence of calcium in the samples from either the control groups (0 } 0.06 ?g/well) or the experimental groups after 24 hours of storage (ANOVA; p>0.05). There was no release of calcium ions, indicating an absence of remineralizing potential. Conclusion: It is concluded that although the experimental resins demonstrated dimensional stability, their high solubility and lack of bioactivity limit their use as remineralizing agents. Formulation adjustments are necessary to improve chemical stability and bioactive potential.